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Reverse Phase Columns Other than ODS

 Reverse Phase Columns (other than ODS)

 

Elution behavior dependent on alkyl chain length

 

In reverse-phase chromatography, retention due to hydrophobicity generally depends directly on the carbon number of the stationary phase. The degree of retention due to hydrophobicity of the stationary phase can generally be listed in descending order by column type as ODS>C8>C4>TMS. Stationary phases with low hydrophobicity can be used effectively to reduce analysis time for samples having too strong of a retention on ODS. Stationary  phases with low hydrophobicity are also useful for samples that are slightly soluble in organic solvents and need to be analyzed with mobile phase containing a low concentration of organic solvents.

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